Browsing by Author "Adesida, S. A."

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    Chemical composition and healing potential of essential oil of Dennettia tripetala on methicillin-resistant Staphylococcus aureus: infected wound model
    (Springer Science+Business Media (Springer Nature), 2022) Adesida, S. A.; Iyebeye, M. I.; Aina, O. O; Peters, R. F.; Ezeaku, C. C.; Amosun, E. A.
    Background: Dennettia tripetala (Pepperfruit) is an important medicinal plant in some West African communities. This study was designed to examine thè antibacterial properties and wound-healing abilities of D. tripetala essential oil on mice with methicillin-resistant Staphylococcus aureus (MRSA) infections. Gas chromatography-mass spectrom- etry (GC-MS) was used to analyse thè essential oil (EO) extracted by hydro-distillation from thè dried fruits of D. tripetala. MRSA was identified using 30 pg cefoxitin disk, CHROMagar, and Polymerase Chain Reaction. Congo red agar and tube technique were used toassessthe production of biofìlms.The antimicrobial susceptibilityfor both antibiot- ics and essential oil was determined by Kirby Bauer and broth dilution methods. Eleven male mice were used in thè in vivo study, and each animai had wound infection on thè dorsal inter-scapular skin region created with a 6 mm biopsy punch and 50 pi (adjusted to 0.5 McFarland standard) of MRSA. The size ofthe wound and its histological characteris- tics were used to estimate healing rate. Results: The GC-MS investigation ofthe essential oil revealed six compounds, with benzene (2-nitroethyl) being thè most prominent. Out ofthe eighteen (18) isolates examined, 12 MRSA strains were identified using thè three methods for methicillin resistance determination, with about 80% of them being classified as biofilm producers. More than 60% ofthe MRSA isolates were resistant to erythromycin, fusidic acid, gentamicin and trimethoprim/sulfamethoxazole. The essential oil had greater antibacterial activity than thè reference antibiotic, vancomycin.The essential oil had a minimum inhibitory concentration of80 l/ml and a minimum bactericidal concentration of 160 l/ml (v/v). Haema- toxylin and eosin staining revealed that thè skin tissue that had been exposed to D. tripetala essential oil had a thicker epithelial layer, numerousfibroblasts, a build-up of collagen, and many blood cells. Condusions: The results showed that D. tripetala essential oil has powerful anti-staphylococcal properties as well as thè capacity to expedite wound healing.This suggests that D. tripetala essential oil could be a successful candidate for developing a topical agent for wound management.
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    Nasal carriage of methicilliin resistant staphylococcus aureus in livestock and farmworkers in Two Communities in Lagos, Nigeria
    (Israel Veterinary Medical Association (IVMA), Raanana, Israel, 2019) Adesida, S. A.; Oke, A.-O. O.; Amosun, E. A.; Coker, A. O.
    The epidemiology of methicillin resistant Staphylococcus aureus (MRSA) is dynamic and thè associated public health risk is likely to increase in settings where there is dose interaction between humans and animals.This study assessed thè occurrence of antimicrobial resistance among S. aureus from livestock and farm personnel, and estimated methicillin-resistance among thè isolates.Two hundred and fifty (250) nasal specimens were collected ffom sheep, goats, cows and farm personnel who had contact with thè animals in two farms in a sub- urban region within Lagos State. Antibiotic susceptibility test was performed using disk diffusion method. The presence of mecA and mecC gene was determined by PCR. S. aureus was recovered from 141 (56.4%) of thè 250 nasal samples analyzed: 32 (22.7%) from cows, 25 (17.7%) from sheep, 32 (22.7%) from pigs, 24 (17.0%) from goats and 28 (19.9 %) from farm workers. Ten isolates, consisting of 4 from cows (8%), 3 from pigs (6%), and 3 from farm personnel (6%), were positive for MRSA. The human-MRSA were recovered from pig workers (2) and a cow farm worker (1). All MRSA strains were resistant to at least three different antimicrobial categories. The MSSA strains were dassified into thirteen antibiotypes of various subtypes. mecC- MRSA was not detected. The high frequency of S. aureus with phenotypic multidrug resistance traits encountered in this study presents a major public health issue. Thus, practices directed at minimizing thè burden of antimicrobial resistance in farm animals should be initiated.