Browsing by Author "Arowolo, O. A."

Now showing 1 - 10 of 10

Draft genome sequence of a bovine enterovirus isolate recovered from sewage in Nigeria
(American Society for Microbiology, Washington DC, 2018) Faleye, T. O. C.; Adewumi, O. M.; Olayinka, O. A.; Donbraye, E.; Oluremi, B.; George, U. E.; Arowolo, O. A.; Omoruyi, E. C.; Ifeorah, M. I.; Oyero, A. O.; Adenijia, J. A.
We describe the draft genome of a bovine enterovirus (EV) isolate recovered from sewage in Nigeria. This isolate replicates on both RD and L20B cell lines but is negative for all EV screens in use by the Global Poliovirus Eradication Initiative (GPEI). It contains 7,368 nucleotides (nt) with 50.2% G + C content and an open reading frame (ORF) with 6,525 nt (2,174 amino acids).
Draft genome sequence of a bovine enterovirus isolate recovered from sewage in Nigeria
(American Society for Microbiology (ASM), 2018) Faleye, T. O. C; Adewumi, O. M.; Olayinka, O. A.; Donbraye, E; Oluremi, B.; George, U. E.; Arowolo, O. A.; Omoruyi, E. C.; Ifeorah, M. I.; Oyero, A . O.; Adeniji, J. A.
We describe the draft genome of a bovine enterovirus (EV) isolate recovered from sewage in Nigeria. This isolate replicates on both RD and L20B cell lines but is negative for all EV screens in use by the Global Poliovirus Eradication Initiative (GPEI). It contains 7,368 nucleotides (nt) with 50.2% G C content and an open reading frame (ORF) with 6,525 nt (2,174 amino acids).
Draft Genome Sequence of Mycoplasma arginini Strain NGR_2017
(Merican Society for Microbiology (ASM), 2018-06-28) Adeniji, J.A.; Faleye, T. O. C.; Adewu , M. O.; Olayinka O. A.; Donbraye, E.; Oluremi, B.; George,a U. E.; Arowolo, O. A.; Omoruy, E. C.; Ifeorah, M. I.; Akande, A.
We present the draft genome of Mycoplasma arginini strain NGR_2017. This strain was recovered in Nigeria from cell culture in 2017. The assembly contains 620,555 bp in 12 contigs. It contains 561 coding sequences, 34 RNAs (29 tRNAs, 4rRNAs, and 1 transfer-messenger RNA [tmRNA]), and a_26-kb integrative and conjugative element.
Draft genome sequence of mycoplasma arginini strain NGR_2017
(American Society for Microbiology, Washington DC, 2018-06) Adeniji, J. A.; Faleye, T. O. C.; Adewumi, O. M.; Olayinka, O. A.; Donbraye, E.; Oluremi, B; George, U. E.; Arowolo, O. A.; Omoruyi, E. C.; Ifeorah, M.; Akandeh, A.
We present the draft genome of Mycoplasma arginini strain NGR_2017. This strain was recovered in Nigeria from cell culture in 2017. The assembly contains 620,555 bp in 12 contigs. It contains 561 coding sequences, 34 RNAs (29 tRNAs, 4 rRNAs, and 1 transfer-messenger RNA [tmRNA]), and a_26-kb integrative and conjugative element.
Faecal antibiotic resistome of Nigerian chimpanzees from a wildlife sanctuary in Cross-River State, Nigeria
(Faecal antibiotic resistome of Nigerian chimpanzees from a wildlife sanctuary in Cross-River State, Nigeria, 2021-06) George, U. E.; Arowolo, O. A.; Olayinka, O. A.; Ifeorah, I. M.; Faleye, T. O. C.; Oluremi, B.; Oragwa, A. O.; Omoruyi, E. C.; Udoh, E. E.; Osasona, O. G.; Donbraye, E.; Adeniji, O. P.; Adewumi, O. M.; Adeniji, J. A.
Out of prescription, indiscriminate use, misuse and abuse of antibiotic administration and/or usage in both humans and farm animals have led to a consistent interference and contamination of biomes and ecobiomes. These resultantly give platforms for positive selection of resistant pathogens and high levels of Antibiotic Resistance Genes (ARGs). We examined Nigerian Chimpanzees in Boki Afi Mountain Wildlife Sanctuary, Cross-River State, Nigeria, to detect ARGs. Faecal samples from 15 Chimpanzees in pristine enclosures of Non-Human Primates in the Wildlife Sanctuary were analyzed. All faecal samples were pooled, then resuspended in phosphate-buffered saline. Subsequently, nucleic acid was extracted from the suspension and Illumina sequencing performed. ARGs in the raw reads were determined and assembled using the KmerResistance tool v2.2. From the 2,763,954 reads generated, 14 ARGs with statistically significant reads were identified. Precisely, 90.5% (12/14) of the ARGs detected target drugs that inhibit translation, of which 66.6% (8/12) were tetracycline resistance (TC-r) genes, while remaining 9.5% (2/14) inhibit cell wall synthesis (cfxA3_1 and cfxA6_1). Eight (aph(3’)-III_1, cfxA3_1, cfxA6_1, erm(B)_10, tet(Q)_1, tet(Q)_2, tet(Q)_4, tet(W)_5) of the ARGs detected were predicted to be plasmid-borne. We report using a cultivation-independent approach the presence of ARGs in Nigerian Chimpanzees. Findings suggest Nigerian Chimpanzees may constitute a hitherto overlooked source of antibiotic resistance in the environment. These ARGs may have been exchanged with handlers and rural dwellers around the Sanctuary. Surveillance of sympatric human faecal and environmental microbiota and their resistomes at the Wildlife Sanctuary are merited to inform public health interventions and decrease ARGs dissemination.
Faecal antibiotic resistome of Nigerian chimpanzees from a wildlife sanctuary in cross-river state, Nigeria
(Nexus Academic Publishers (NAP), 2021) George, U. E.; Arowolo, O. A.; Olayinka, O. A.; Ifeorah, I. M.; Faleye, T. O. C.; Oluremi, B.; Oragwa, A.O.; Omoruyi, E. C.; Udoh, E. E.; Osasona, O. G.; Donbraye, E.; Adeniji, O. P.; Adewumi, O. M.; Adeniji, J. A.
"Out of prescription, indiscriminate use, misuse and abuse of antibiotic administration and/or usage in both humans and farm animals have led to a consistent interference and contamination of biomes and ecobiomes. These resultantly give platforms for positive selection of resistant pathogens and high levels of Antibiotic Resistance Genes (ARGs). We examined Nigerian Chimpanzees in Boki Afi Mountain Wildlife Sanctuary, Cross-River State, Nigeria, to detect ARGs. Faecal samples from 15 Chimpanzees in pristine enclosures of Non-Human Primates in the Wildlife Sanctuary were analyzed. All faecal samples were pooled, then resuspended in phosphate-buffered saline. Subsequently, nucleic acid was extracted from the suspension and Illumina sequencing performed. ARGs in the raw reads were determined and assembled using the KmerResistance tool v2.2. From the 2,763,954 reads generated, 14 ARGs with statistically significant reads were identified. Precisely, 90.5% (12/14) of the ARGs detected target drugs that inhibit translation, of which 66.6% (8/12) were tetracycline resistance (TC-r) genes, while remaining 9.5% (2/14) inhibit cell wall synthesis (cfxA3_1 and cfxA6_1). Eight (aph(3’)-III_1, cfxA3_1, cfxA6_1, erm(B)_10, tet(Q)_1, tet(Q)_2, tet(Q)_4, tet(W)_5) of the ARGs detected were predicted to be plasmid-borne. We report using a cultivation-independent approach the presence of ARGs in Nigerian Chimpanzees. Findings suggest Nigerian Chimpanzees may constitute a hitherto overlooked source of antibiotic resistance in the environment. These ARGs may have been exchanged with handlers and rural dwellers around the Sanctuary. Surveillance of sympatric human faecal and environmental microbiota and their resistomes at the Wildlife Sanctuary are merited to inform public health interventions and decrease ARGs dissemination."
Fecal antibiotic resistome of pigs from a small-scale piggery in Ibadan, South-West Nigeria
(ResearchersLinks, 2021) Olayinka, O. A.; Faleye, T. O. C.; Omotosho, O. O.; Odukaye, O. A.; Oluremi, B.; Ibitoye, I. H.; Ope-Ewe, O. O.; George, U. E.; Arowolo, O. A.; Ifeorah, I. M.; Omoruyi, E. C.; Donbraye, E.; Adeniji, O. P.; Adewumi, O. M.; Adeniji, J. A.
This study was designed to sample the fecal Resistome of Pigs from a small-scale Piggery in Ibadan, South-West Nigeria. Three fecal pellets were randomly picked from the floor of unit pens containing a minimum of three pigs per unit in selected piggery in Ibadan, Nigeria. The samples were pooled and resuspended in phosphate buffered saline. The suspension was then subjected to nucleic acid extraction, cDNA synthesis and Illumina sequencing. Antibiotic Resistance Genes (ARGs) in the raw reads were determined and assembled using the Kmer Resistance tool v2.2. From the 2,974,257 reads generated, 21 ARGs with statistically significant reads were identified. Almost all targeted broad-spectrum antibiotics with over 50% targeting Tetracyclines. Five (ant(6)-Ia_3, tet(40)_1, tet(Q)_1, tet(W)_5 and tet(O/W)_4) of the ARGs were predicted to be plasmid-borne. Our findings show that the Swine industry in the region might be both a mixing pot and reservoir of ARGs. It is therefore crucial that effort is made to educate the stakeholders on the importance of good antibiotics stewardship.
Near-complete genome sequence of an enterovirus species F isolate recovered from sewage in Nigeria
(American Society for Microbiology, Washington DC, 2020) Faleye, T. O. C.; Ifeorah, M. I.; Olayinka, O. A.; Oluremi, B.; George, U. E.; Arowolo, O. A.; Omoruyi, E. C.; Donbraye, E.; Oyero, A. O.; Adewumi, O. M.; Adeniji, J. A.
Here, we describe the near-complete genome of an enterovirus F (EV-F) isolate from Nigeria. The obtained sequence was 7,378 nucleotides (nt) long and encodes 2 open reading frames (ORFs), an upstream ORF (uORF; 56 amino acids [aa]) and a polyprotein ORF (ppORF; 2,167 aa). Both ORFs overlap but are in different reading frames, with the uORF in a +1 reading frame relative to the ppORF.
Near-complete genome sequence of an enterovirus species fisolate recovered from sewage in Nigeria
(American Society for Microbiology (ASM), 2021) Faleye, T. O. C.; Ifeorah, M. I.; Olayinka, O. A.; Oluremi, B.; George, U. E.; Arowolo, O. A.; Omoruyi, E. C.; Donbraye, E.; Oyero, A. O.; Adewumi, O. M.; Adeniji, J.A.
Here, we describe the near-complete genome of an enterovirus F (EV-F) isolate from Nigeria. The obtained sequence was 7,378 nucleotides (nt) long and encodes 2 open reading frames (ORFs), an upstream ORF (uORF; 56 amino acids [aa])and a polyprotein ORF (ppORF; 2,167 aa). Both ORFs overlap but are in different reading frames, with the uORF in a _1 reading frame relative to the ppORF.
Small bowel responses to enteral honey and glutamine administration following massive small bowel resection in rabbit
(2008) Eyarefe, O.D.; Emikpe, B. O.; Arowolo, O. A.
The trophic effects of honey and glutamine in the healing and adaptation of the small bowel following intestinal resection were studied in some Nigerian non-descript breeds of rabbits. Nine rabbits of mixed sexes with mean body weight of 1.45 ±0.55kg were used. They were randomised into three treatment groups following 50% small bowel resection. Group A rabbits were placed on oral honey treatment. Group B on oral glutamine and group C on normal saline (control). All groups exhibited signs of small bowel adaptation (glutamine(B) honey (A) control (C) at the end of the experiment (4 weeks) with oral glutamine showing the best overall effects on intestinal mucosal growth and adaptation evidenced by significant increase (P<0.05) in residual bow'el length (37.3%), villi width (20.0%), crypt depth (113.3%) and a non significant increase in villus height (33.3%) and cellular mass (10.2%). Honey showed a better effect than control with a significant increase (P<0.05) in villi width (18.2%), crypts depth (66.7%) and cellular mass (33.9%) and a non significant increase in gross residual bow'el length (24.6%), and villus height (30.5%). Our result shows that honey and glutamine have trophic effects on bowel mucosa healing and hyperplasia and have potential therapeutic effects on massive bowel resection in humans