Browsing by Author "Kotun, B. C."

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Antimicrobial susceptibility of Pseudomonas aeruginosa isolated from contact lenses of some selected individuals in Caleb University, Imota, Lagos State.
(Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, 2019) Okunye, O. L.; Idowu, P. A.; Kotun, B. C.; Kolade, T. T.
Contact lenses are ocular prosthetic devices used by over 150 million people worldwide, and they can be worn to correct vision, for cosmetic, or therapeutic reasons. This epidemiological study was performed to determine the prevalence of Pseudomonas aeruginosa on contact lenses of selected students in Caleb University, Imota, Lagos State. A swab of 100 contact lenses from selected volunteers comprising 43 males and 57 females were collected for microbiological examination. Sterile swab sticks moistened with sterile distilled water were used to aseptically swab the lenses and transferred into nutrient broth followed by incubation for 5 hours. Thereafter, the resultant broth culture was subcultured on to cetrimide nutrient agar and incubated at 370C for 24 hours for the isolation of Pseudomonas aeruginosa. A total of 30 (30%) of the contact lenses sampled were found to have Pseudomonas aeruginosa, of which 13(43.3%) of the isolates were pigmented and 17(56.7%) were non-pigmented. Eighty percent (80%) of the isolates were susceptible to ciprofloxacin, 75% to gentamicin while 50% were susceptible to ofloxacin. The isolates were 90% resistant to nitrofurantoin, 75% resistant to augmentin and 100% to ampicillin. Ciprofloxacin and gentamicin were found to be effective against Pseudomonas aeruginosa in this study and could be of therapeutic relevance for the management of contact lenses mediated infections.
Antimicrobial susceptibility pattern of Pseudomonas aeruginosa isolated from contact lenses of some selected individuals in Calen university, Imota, Lagos State
(Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, 2019) Okunye, O. L.; Idowu, P. A.; Kotun, B. C.; Kolade, T. T.
Contact lenses are ocular prosthetic devices used by over 150 million people worldwide, and they can be worn to correct vision, for cosmetic, or therapeutic reasons. This epidemiological study was performed to determine the prevalence of Pseudomonas aeruginosa on contact lenses of selected students in Caleb University, Imota, Lagos State. A swab of 100 contact lenses from selected volunteers comprising 43 males and 57 females were collected for microbiological examination. Sterile swab sticks moistened with sterile distilled water were used to aseptically swab the lenses and transferred into nutrient broth followed by incubation for 5 hours. Thereafter, the resultant broth culture was subcultured on to cetrimide nutrient agar and incubated at 370C for 24 hours for the isolation of Pseudomonas aeruginosa. A total of 30 (30%) of the contact lenses sampled were found to have Pseudomonas aeruginosa, of which 13(43.3%) of the isolates were pigmented and 17( 56.7%) were non-pigmented. Eighty percent (80%) of the isolates were susceptible to ciprofloxacin, 75% to gentamicin while 50% were susceptible to ofloxacin. The isolates were 90% resistant to nitrofurantoin, 75% resistant to augmentin and 100% to ampicillin. Ciprofloxacin and gentamicin were found to be effective against Pseudomonas aeruginosa in this study and could be of therapeutic relevance for the management of contact lenses mediated infections.
Evaluation of antifungal activity of lactic acid bacteria from locally sourced yoghurts on isolates of Candida albicans from cases of vaginitis
(Cameroon Academy of Sciences, 2024) Okunye, O. L.; Idowu, P. A.; Babalola, C. O.; Kotun, B. C.; Ayedun, J. S.; Adesanya, A. A.; Kolade, T. T.; Iloka, B. C.
This study assessed antifungal activity of lactic acid bacteria from locally made yoghurts on isolates of candida albicans from cases of vaginitis. Locally sourced yoghurt samples were cultured on MRS agar and incubated anaerobically at optimum temperature for the isolation of Lactic Acid Bacteria. The pH and total viable counts parameters of the isolates from the samples were determined. Isolates of Candida albicans from cases of vaginitis collected were biochemically confirmed after SDA plated. The isolates were exposed to antifungal agents with varied concentrations and thereafter, challenged with Lactic Acid Bacteria laden yoghurt samples prepared in varied concentration on MRS agar over-layered with SDA. Plasmid DNA of the LAB isolates were determined. The isolates of Candida albicans exhibited 100% resistance to conventional antifungal agents exposed with the exception of Candida albicans 1 that were susceptible to all the antifungal agents while Candida albicans 4 was susceptible to amphotericin B, fluconazole, and nystatin. Three(3) of the four (4) isolates of Candida albicans challenged with Habib (LABHAY) and Tunik (LAB TY ) yoghurt were 100% susceptible the LAB laden yoghurt samples while Candida albicans isolates 3 (Ca3) were (100%) resistant to LABHAY and LAB TY. Of the four (4) isolates of Candida albicans were(100%) susceptible to Cedaar (LABCY) and Fan milk(LABFM) yoghurt and while Candida albicans isolates number 3 (Ca3) exhibited resistance to LABCY and LABFM. Lactic Acid Bacteria in this study, exhibited remarkable antifungal activity on Candida albicans isolates from cases of vaginitis. This property could be exploited as a bio-protective and therapeutic option, for cases of Candida albicans and fungi with related features.
Evaluation of antifungal activity of lactic acid bacteria from locally sourced yoghurts on isolates of Candida Albicans from cases of vaginitis
(Cameroon Academy of Sciences (CAS), 2024) Okunye, O. L.; Idowu, P. A.; Babalola, C. O.; Kotun, B. C.; Ayedun, J. S.; Adesanya, A. A.; Kolade, T. T.; Iloka, B. C.
This study assessed antifungal activity of lactic acid bacteria from locally made yoghurts on isolates of candida albicans from cases of vaginitis. Locally sourced yoghurt samples were cultured on MRS agar and incubated anaerobically at optimum temperature for the isolation of Lactic Acid Bacteria. The pH and total viable counts parameters of the isolates from the samples were determined. Isolates of Candida albicans from cases of vaginitis collected were biochemically confirmed after SDA plated. The isolates were exposed to antifungal agents with varied concentrations and thereafter, challenged with Lactic Acid Bacteria laden yoghurt samples prepared in varied concentration on MRS agar overlayered with SDA. Plasmid DNA of the LAB isolates were determined. The isolates of Candida albicans exhibited 100% resistance to conventional antifungal agents exposed with the exception of Candida albicans 1 that were susceptible to all the antifungal agents while Candida albicans 4 was susceptible to amphotericin B, fluconazole, and nystatin. Three(3) of the four (4) isolates of Candida albicans challenged with Habib (LABHAY) and Tunik(LAB TY ) yoghurt were 100% susceptible the LAB laden yoghurt samples while Candida albicans isolates 3 (Ca3) were (100%) resistant to LABHAY and LAB TY. Of the the four(4) isolates of Candida albicans were(100%) susceptible to Cedaar (LABCY) and Fan milk(LABFM) yoghurt and while Candida albicans isolates number 3 (Ca3) exhibited resistance to LABCY and LABFM. Lactic Acid Bacteria in this study, exhibited remarkable antifungal activity on Candida albicans isolates from cases of vaginitis. This property could be exploited as a bio-protective and therapeutic option, for cases of Candida albicans and fungi with related features.