Browsing by Author "Olowookorun, T.O."

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    Biosynthesis, characterization and antivenom activities of Moringa oleifera silver nanoparticles: an experimental approach
    (Zoological Society of Nigeria, 2023) Adeyi, A.O.; Olowookorun, T.O.; Ajisebiola, B.S.; Labulo, H.A.; Adeyi, O.E.; Ibrahim, H.
    Moringa oleifera has been previously established to possess neutralizing potentials against Echis ocellatus venom. This study however, investigated the bioefficacy of silver nanoparticles biosynthesized from M. oleifera leaf extract aimed at improving its bioactivity against E. ocellatus venom-induced toxicities using in vivo and in vitro methods. The intrinsic characteristics of the produced M. oleifera-Silver nanoparticles (MO-AgNPs) were carried out using energy dispersive X-ray, X-ray diffraction, scanning electron microscopy, transmission electron microscopy and Fourier-transform infrared spectroscopy. Twenty-five male Wistar rats divided randomly into five groups (n=5) were used for the antivenom study. Group 1 received saline while groups 2 to 5 were envenomed intraperitoneally with 0.22mg/kg (LD50) of E. ocellatus venom. Group 2 was left untreated while groups 3 to 5 were treated with 0.2ml of antivenom, 5 and 10mg/kg MO-AgNPs post-envenomation, respectively. Blood and tissue of treated rats were analyzed for heamatological parameters and histopathology, respectively. The MO-AgNPs formation was confirmed with a colour change from light brown to yellowish-brown with maximum SPR band at 420nm from UV-Vis analysis, indicating a reflection of the bio-reduction of Ag+ to Ag0. The Transmission electron micrographs showed well dispersed spherical AgNPs with average particle size of 15.7nm. Treatment with MO-AgNPs caused a significant improvement of acute anemia, leucopenia and thrombocytopenia induced by the venom in the envenomed treated rats. Also, MO-AgNPs inhibited the haemorrhagic, haemolytic and anticoagulant activities of the venom. Tissue lesions observed in heart of envenomed untreated rats were attenuated after treatment with MO-AgNPs. The biosynthesized MO-AgNPs exhibited potent neutralizing potentials than M. oleifera crude extract against E. ocellatus venom toxicities.