Scholarly works in Pharmaceutical Microbiology
Permanent URI for this collectionhttps://repository.ibadanedu.com/handle/123456789/394
Browse
5 results
Search Results
Item Antibacterial activity against clinical isolates of salmonella enterica serovar paratyphi and brine shrimp lethality assay of trichilia megalantha harms and trichilia welwitschii C. DC(Ethiopian Pharmaceutical Journal, 2020) Idowu, P. A.; Adegbenle, T. A.Increasing resistance of typhoidal Salmonella enterica to conventional antibiotics has caused more cases of typhoid, therapeutic failure, morbidity and mortality; creating the need to search for new and effective antimicrobial agents from medicinal plants. The present study aimed to detect antisalmonella activity and cytotoxicity (safety) status of the stem bark and leaves of two Nigerian medicinal plants, Trichilia megalantha and Trichilia welwitschii. Nine clinical isolates of Salmonella paratyphi, whose antibiogram were determined by Kirby-Bauer disc diffusion method were used. Antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the clinical isolates were done by agar cup diffusion and agar dilution methods, respectively. Acute toxicity of the extracts was determined using brine shrimp lethality assay (BSLA). All the nine isolates of Salmonella paratyphi were resistant to ß-lactam antibiotics (augmentin, cefuroxime, ceftazidime and ampicillin) but susceptible to fluoroquinolones (ciprofloxacin, ofloxacin), nitrofurantoin and gentamicin. The crude extracts of the two plants elicited activity against the nine clinical isolates with the bark extracts being more active than the leaf extracts. I. welwitschi was slightly less active than T. megalantha. The MIC and MBC ranged 1.25 -5.0 mg/ ml and 2.5-10 mg/ml, respectively for the plants. The antisalmonella activity of methanolic extracts of both plants were found to be less than that of ciprofloxacin and ofloxacin. With modal cytotoxicity values of 400- 500 ug/ml, the plant parts were considered nontoxic. Therefore, T. megalantha and T. welwitschii could provide a potential source of antibacterial agent(s) for the treatment of Salmonella paratyphoid infections.Item Antibacterial activity against clinical isolates of salmonella enterica serovar paratyphi and brine shrimp lethality assay of trichilia megalantha harms and trichilia welwitschii C. DC(Ethiopian Pharmaceutical Journal, 2020) Idowu, P. A.; Adegbenle, T. A.Increasing resistance of typhoidal Salmonella enterica to conventional antibiotics has caused more cases of typhoid, therapeutic failure, morbidity and mortality; creating the need to search for new and effective antimicrobial agents from medicinal plants. The present study aimed to detect antisalmonella activity and cytotoxicity (safety) status of the stem bark and leaves of two Nigerian medicinal plants, Trichilia megalantha and Trichilia welwitschii. Nine clinical isolates of Salmonella paratyphi, whose antibiogram were determined by Kirby-Bauer disc diffusion method were used. Antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the clinical isolates were done by agar cup diffusion and agar dilution methods, respectively. Acute toxicity of the extracts was determined using brine shrimp lethality assay (BSLA). All the nine isolates of Salmonella paratyphi were resistant to ß-lactam antibiotics (augmentin, cefuroxime, ceftazidime and ampicillin) but susceptible to fluoroquinolones (ciprofloxacin, ofloxacin), nitrofurantoin and gentamicin. The crude extracts of the two plants elicited activity against the nine clinical isolates with the bark extracts being more active than the leaf extracts. I. welwitschi was slightly less active than T. megalantha. The MIC and MBC ranged 1.25 -5.0 mg/ ml and 2.5-10 mg/ml, respectively for the plants. The antisalmonella activity of methanolic extracts of both plants were found to be less than that of ciprofloxacin and ofloxacin. With modal cytotoxicity values of 400- 500 ug/ml, the plant parts were considered nontoxic. Therefore, T. megalantha and T. welwitschii could provide a potential source of antibacterial agent(s) for the treatment of Salmonella paratyphoid infections.Item Antibacterial activity against clinical isolates of salmonella enterica serovar paratyphi and brine shrimp lethality assay of trichilia megalantha harms and trichilia welwitschii C. DC(Ethiopian Pharmaceutical Journal, 2020) Idowu, P. A.; Adegbenle, T. A.Increasing resistance of typhoidal Salmonella enterica to conventional antibiotics has caused more cases of typhoid, therapeutic failure, morbidity and mortality; creating the need to search for new and effective antimicrobial agents from medicinal plants. The present study aimed to detect antisalmonella activity and cytotoxicity (safety) status of the stem bark and leaves of two Nigerian medicinal plants, Trichilia megalantha and Trichilia welwitschii. Nine clinical isolates of Salmonella paratyphi, whose antibiogram were determined by Kirby-Bauer disc diffusion method were used. Antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the clinical isolates were done by agar cup diffusion and agar dilution methods, respectively. Acute toxicity of the extracts was determined using brine shrimp lethality assay (BSLA). All the nine isolates of Salmonella paratyphi were resistant to ß-lactam antibiotics (augmentin, cefuroxime, ceftazidime and ampicillin) but susceptible to fluoroquinolones (ciprofloxacin, ofloxacin), nitrofurantoin and gentamicin. The crude extracts of the two plants elicited activity against the nine clinical isolates with the bark extracts being more active than the leaf extracts. I. welwitschi was slightly less active than T. megalantha. The MIC and MBC ranged 1.25 -5.0 mg/ ml and 2.5-10 mg/ml, respectively for the plants. The antisalmonella activity of methanolic extracts of both plants were found to be less than that of ciprofloxacin and ofloxacin. With modal cytotoxicity values of 400- 500 ug/ml, the plant parts were considered nontoxic. Therefore, T. megalantha and T. welwitschii could provide a potential source of antibacterial agent(s) for the treatment of Salmonella paratyphoid infections.Item Antibacterial activity against clinical isolate of Salmonella entrica serovar Paratyphi and Brine Shrimp Lethality Assay of Trichilia megalantha harms and Trichilia welwitschii C.DC(Ethiopian Pharmaceutical Association, 2020) Idowu, P. A.; Adegbenle, T. A.Increasing resistance of typhoidal Salmonella enterica to conventional antibiotics has caused more cases of typhoid, therapeutic failure, morbidity and mortality; creating the need to search for new and effective antimicrobial agents from medicinal plants. The present study aimed to detect antisalmonella activity and cytotoxicity (safety) status of the stem bark and leaves of two Nigerian medicinal plants, Trichilia megalantha and Trichilia welwitschii. Nine clinical isolates of Salmonella paratyphi, whose antibiogram were determined by Kirby-Bauer disc diffusion method were used. Antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the clinical isolates were done by agar cup diffusion and agar dilution methods, respectively. Acute toxicity of the extracts was determined using brine shrimp lethality assay (BSLA). All the nine isolates of Salmonella paratyphi were resistant to p-lactam antibiotics (augmentin, cefuroxime, ceftazidime and ampicillin) but susceptible to fluoroquinolones (ciprofloxacin, ofloxacin), nitrofurantoin and gentamicin. The crude extracts of the two plants elicited activity against the nine clinical isolates with the bark extracts being more active than the leaf extracts. T. welwitschii was slightly less active than T. megalantha. The MIC and MBC ranged 1.25 - 5.0 mg/ ml and 2.5 - 10 mg/ml, respectively for the plants. The antisalmonella activity of methanolic extracts of both plants were found to be less than that of ciprofloxacin and ofloxacin. With modal cytotoxicity values of 400 - 500 pg/ml, the plant parts were considered nontoxic. Therefore, T. megalantha and T. welwitschii could provide a potential source of antibacterial agent(s) for the treatment of Salmonella paratyphoid infections.Item Isolation of 3-(4-hydroxyphenyl) methylpropenoate and bioactivity evaluation of Gomphrena celosioides Extracts(IfADo - Leibniz Research Centre for Working Environment and Human Factors, 2010) Dosumu, O. O.; Idowu, P. A.; Onocha, P. A.; Ekundayo, O.The efficacy of Gomphrena celosioides extracts in traditional medicine in the treatment of infectious diseases was evaluated by biological assays. The bioactivities of the extracts of this plant were tested against organisms. The ethyl acetate and methanol extracts of the plant dis played inhibition activities on Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi. Methanol extract was active against Candida albicans, Aspergillus niger and Trichophyton species with diameter zones of inhibition between 14 and 20 mm. Fractionation of the methanol extract produced 3-(4-hydroxyphenyl) methylpropenoate with mild antimicrobial activity against the test microorganisms. The anthelmintic assay showed ethyl acetate and methanol extracts to be active against Fasciola gigantica, Taenia solium and Pheretima pasthuma. Ethyl acetate was the most toxic of the extracts causing paralysis of Taenia solium within 15 minutes and the death of Fasciola gigantica within 20 minutes of application. The brine shrimp assay gave an LC50 of 52.15 and 77.98 µg/ml on hexane and methanol extracts respectively. The result of this work corroborated the folkloric use of Gomphrena celosioides in the treatment of infectious diseases.